Ebola virus proteins NP, VP35, and VP24 are essential and sufficient to mediate nucleocapsid transport.

ABSTRACT: The intracytoplasmic movement of nucleocapsids is a crucial step in the life cycle of enveloped viruses. Determination of the viral components necessary for viral nucleocapsid transport competency is complicated by the dynamic and complex nature of nucleocapsid assembly and the lack of appropriate model systems. Here, we established a live-cell imaging system based on the ectopic expression of fluorescent Ebola virus (EBOV) fusion proteins, allowing the visualization and analysis of the movement of EBOV nucleocapsid-like structures with different protein compositions. Only three of the five EBOV nucleocapsid proteins-nucleoprotein, VP35, and VP24-were necessary and sufficient to form transport-competent nucleocapsid-like structures. The transport of these structures was found to be depende

SUBMITTER: Takamatsu Y 

PROVIDER: S-EPMC5798334 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature