Ultrafast imaging of cell elasticity with optical microelastography.

ABSTRACT: Elasticity is a fundamental cellular property that is related to the anatomy, functionality, and pathological state of cells and tissues. However, current techniques based on cell deformation, atomic force microscopy, or Brillouin scattering are rather slow and do not always accurately represent cell elasticity. Here, we have developed an alternative technique by applying shear wave elastography to the micrometer scale. Elastic waves were mechanically induced in live mammalian oocytes using a vibrating micropipette. These audible frequency waves were observed optically at 200,000 frames per second and tracked with an optical flow algorithm. Whole-cell elasticity was then mapped using an elastography method inspired by the seismology field. Using this approach we show that the elasticity of

SUBMITTER: Grasland-Mongrain P 

PROVIDER: S-EPMC5798341 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature