Real-time Analysis of Auxin Response, Cell Wall pH and Elongation in Arabidopsis thaliana Hypocotyls.
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ABSTRACT:
SUBMITTER: Li L
PROVIDER: S-EPMC5798589 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Publications
Real-time Analysis of Auxin Response, Cell Wall pH and Elongation in <i>Arabidopsis thaliana</i> Hypocotyls.
Li Lanxin L Krens S F Gabriel SFG Fendrych Matyáš M Friml Jiří J
Bio-protocol 20180101 1
The rapid auxin-triggered growth of the <i>Arabidopsis</i> hypocotyls involves the nuclear TIR1/AFB-Aux/IAA signaling and is accompanied by acidification of the apoplast and cell walls (Fendrych <i>et al</i>., 2016). Here, we describe in detail the method for analysis of the elongation and the TIR1/AFB-Aux/IAA-dependent auxin response in hypocotyl segments as well as the determination of relative values of the cell wall pH. ...[more]
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