Dynamics of adhesion molecule domains on neutrophil membranes: surfing the dynamic cell topography.

ABSTRACT: Lateral organization and mobility of adhesion molecules play a significant role in determining the avidity with which cells can bind to target cells or surfaces. Recently, we have shown that the lateral mobility of the principal adhesion molecules on neutrophils is lower for rolling associated adhesion molecules (RAAMs: L-selectin and PSGL-1) than for β2 integrins (LFA-1 and Mac-1). Here we report that all four adhesion molecules exhibit distinct punctate distributions that are mobile on the cell surface. Using uniform illumination image correlation microscopy, we measure the lateral mobility of these topologically distinct domains. For all four molecules, we find that diffusion coefficients calculated from domain mobility agree with measurements we made previously using fluorescence recov

SUBMITTER: Gaborski TR 

PROVIDER: S-EPMC5798999 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature