Neuroprotection of the hypoxic-ischemic mouse brain by human CD117⁺CD90⁺CD105⁺ amniotic fluid stem cells.

ABSTRACT: Human amniotic fluid contains two morphologically-distinct sub-populations of stem cells with regenerative potential, spindle-shaped (SS-hAFSCs) and round-shaped human amniotic fluid stem cells (RS-hAFSCs). However, it is unclear whether morphological differences correlate with functionality, and this lack of knowledge limits their translational applications. Here, we show that SS-hAFSCs and RS-hAFSCs differ in their neuro-protective ability, demonstrating that a single contralateral injection of SS-hAFSCs into hypoxic-ischemic P7 mice conferred a 47% reduction in hippocampal tissue loss and 43-45% reduction in TUNEL-positive cells in the hippocampus and striatum 48 hours after the insult, decreased microglial activation and TGFβ1 levels, and prevented demyelination. On the other hand, RS-

SUBMITTER: Corcelli M 

PROVIDER: S-EPMC5799160 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature