Effect of Melatonin on the stability and expression of reference genes in Catharanthus roseus.

ABSTRACT: The role of Melatonin in influencing diverse genes in plants has gained momentum in recent years and many reports have employed qRT-PCR for their quantification. Relative quantification of gene expression relies on accurate normalization of qRT-PCR data against a stably-expressing internal reference-gene. Although researchers have been using commonly available reference-genes to assess Melatonin-induced gene expression, but to-date, there have been no attempts to validate the reference-gene stability under Melatonin-supplementation in planta. In this study, we performed stability assessment of common reference-genes under Melatonin-supplementation and abiotic stress in leaves and seedlings of Catharanthus roseus using geNorm, NormFinder, BestKeeper, ΔCt and RefFinder algorithms. Nine candi

SUBMITTER: Sheshadri SA 

PROVIDER: S-EPMC5799177 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature