Design and production of conjugate vaccines against S. Paratyphi A using an O-linked glycosylation system in vivo.

ABSTRACT: Enteric fever, mainly caused by Salmonella enterica serovar Paratyphi A, remains a common and serious infectious disease worldwide. As yet, there are no licensed vaccines against S. Paratyphi A. Biosynthesis of conjugate vaccines has become a promising approach against bacterial infection. However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine. We successfully generated a recombinant S. Paratyphi A strain with a longer O-polysaccharide chain and transformed the O-lin

SUBMITTER: Sun P 

PROVIDER: S-EPMC5799188 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature