Transcriptional decomposition reveals active chromatin architectures and cell specific regulatory interactions.

ABSTRACT: Transcriptional regulation is tightly coupled with chromosomal positioning and three-dimensional chromatin architecture. However, it is unclear what proportion of transcriptional activity is reflecting such organisation, how much can be informed by RNA expression alone and how this impacts disease. Here, we develop a computational transcriptional decomposition approach separating the proportion of expression associated with genome organisation from independent effects not directly related to genomic positioning. We show that positionally attributable expression accounts for a considerable proportion of total levels and is highly informative of topological associating domain activities and organisation, revealing boundaries and chromatin compartments. Furthermore, expression data alone accu

SUBMITTER: Rennie S 

PROVIDER: S-EPMC5799294 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature