Calycosin Orchestrates Osteogenesis of Danggui Buxue Tang in Cultured Osteoblasts: Evaluating the Mechanism of Action by Omics and Chemical Knock-out Methodologies.

ABSTRACT: Danggui Buxue Tang (DBT), an ancient Chinese herbal decoction commonly used to mitigate menopausal osteoporosis, contains two herbs: Astragali Radix (AR) and Angelicae Sinensis Radix (ASR). The exact efficacy of individual chemical(s) within DBT, or in any herbal mixture, is hard to be revealed. Calycosin and ferulic acid have been reported to be the predominant chemicals found within DBT, and its roles in regulating osteoblastic differentiation have been proposed here. To probe the roles of calycosin and ferulic acid, these chemicals were specifically depleted from the DBT extracts. Here, calycosin-depleted DBT (DBT_Δcal) and ferulic acid-depleted DBT (DBT_Δfa), generated by semi-preparative HPLC, were coupled with RNA-seq and metabolomics analyses to reveal the synerg

SUBMITTER: Gong AGW 

PROVIDER: S-EPMC5799702 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature