Long non-coding RNA expression profiling in the lesional tissue and derived fibroblasts of keloid.

ABSTRACT:

Introduction

Long non-coding RNA (lncRNA) plays a key role in various disorders. However, its role in keloid is still unclear.

Aim

We explored differentially expressed (DE) lncRNAs and mRNAs between keloid tissue (KT)s and normal tissue (NT)s, as well as keloid fibroblast (KFB)s and normal fibroblast (NFB)s, respectively.

Material and methods

We use KTs and NTs from the chest of 5 patients, and 3 pairs of KFBs and NFBs, to perform microarray respectively. Gene ontology and pathway analyses were conducted by online software DAVID (Database for Annotation, Visualization and Integrated Discovery). The validation of targeted lncRNAs were conducted by qRT-PCR in enlarged samples (79 KTs and 21 NTs).

Results

We identified 3680 DE-lncRNAs in tissue essay, and 1231 DE-

SUBMITTER: Yuan C 

PROVIDER: S-EPMC5799763 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature