Hepatic stem cells with self-renewal and liver repopulation potential are harbored in CDCP1-positive subpopulations of human fetal liver cells.

ABSTRACT:

Background

Mature human hepatocytes are critical in preclinical research and therapy for liver disease, but are difficult to manipulate and expand in vitro. Hepatic stem cells (HpSCs) may be an alternative source of functional hepatocytes for cell therapy and disease modeling. Since these cells play an import role in regenerative medicine, the precise characterization that determines specific markers used to isolate these cells as well as whether they contribute to liver regeneration still remain to be shown.

Method

In this study, human HpSCs were isolated from human primary fetal liver cells (FLCs) by flow cytometry using CDCP1, CD90, and CD66 antibodies. The isolated CDCP1⁺CD90⁺CD66^- HpSCs were cultured on dishes coated with type IV collage

SUBMITTER: Zhang RR 

PROVIDER: S-EPMC5800061 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature