Sorcin ablation plus β-adrenergic stimulation generate an arrhythmogenic substrate in mouse ventricular myocytes.

ABSTRACT: Sorcin, a penta-EF hand Ca²⁺-binding protein expressed in cardiomyocytes, is known to interact with ryanodine receptors and other Ca²⁺ regulatory proteins. To investigate sorcin's influence on cardiac excitation-contraction coupling and its role in the development of cardiac malfunctions, we generated a sorcin knockout (KO) mouse model. Sorcin KO mice presented ventricular arrhythmia and sudden death when challenged by acute stress induced by isoproterenol plus caffeine. Chronic stress, which was induced by transverse aortic constriction, significantly decreased the survival rate of sorcin KO mice. Under isoproterenol stimulation, spontaneous Ca²⁺ release events were frequently observed in sorcin KO cardiomyocytes. Sorcin KO hearts of adult, but not young m

SUBMITTER: Chen X 

PROVIDER: S-EPMC5800963 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature