Functional interrogation and mining of natively paired human V_H:V_L antibody repertoires.

ABSTRACT: We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (V_H:V_L) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly neutralizing antibodies (bNAbs) from an HIV-1 slow progressor and high-affinity neutralizing antibodies against Ebola virus glycoprotein and influenza hemagglutinin.

SUBMITTER: Wang B 

PROVIDER: S-EPMC5801115 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature