Purified horse milk exosomes contain an unpredictable small number of major proteins.

ABSTRACT: Exosomes are 40-100 nm nanovesicles containing RNA and different proteins. Exosomes containing proteins, lipids, mRNAs, and microRNAs are important in intracellular communication and immune function. Exosomes from different sources are usually obtained by combination of centrifugation and ultracentrifugation and according to published data can contain from a few dozens to thousands of different proteins. Crude exosome preparations from milk of eighteen horses were obtained for the first time using several standard centrifugations. Exosome preparations were additionally purified by FPLC gel filtration. Individual preparations demonstrated different profiles of gel filtration showing well or bad separation of exosome peaks and one or two peaks of co-isolating proteins and their complexes. Ac

SUBMITTER: Sedykh SE 

PROVIDER: S-EPMC5801828 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature