Microarray analysis reveals Tmub1 as a cell cycle-associated protein in rat hepatocytes.

ABSTRACT: Transmembrane and ubiquitin-like domain containing protein 1 (Tmub1), formerly known as hepatocyte odd protein shuttling (HOPS) has been recognized as a ubiquitously expressed shuttling protein that moves between the nucleus and cytoplasm in hepatocytes. Tmub1 is involved in liver regeneration and functions as a bridging protein in tumor cell proliferation. To investigate the transcriptional profile and potential biological processes affected by Tmub1 expression in normal rat hepatocytes, microarray and bioinformatics experiments were used to identify 127 mRNAs differentially expressed between Tmub1‑overexpression, Tmub1‑knockdown and normal BRL‑3A cells (fold‑change ≥2.5). The expression levels of 17 key node genes associated with the cell cycle were confirmed by reverse transcription-qua

SUBMITTER: Fu H 

PROVIDER: S-EPMC5802207 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature