Characterization of inorganic phosphate transport in the triple-negative breast cancer cell line, MDA-MB-231.

ABSTRACT:

Background

Recent studies demonstrate that interstitial inorganic phosphate is significantly elevated in the breast cancer microenvironment as compared to normal tissue. In addition it has been shown that breast cancer cells express high levels of the NaPi-IIb carrier (SLC34A2), suggesting that this carrier may play a role in breast cancer progression. However, the biochemical behavior of inorganic phosphate (Pi) transporter in this cancer type remains elusive.

Methods

In this work, we characterize the kinetic parameters of Pi transport in the aggressive human breast cancer cell line, MDA-MB-231, and correlated Pi transport with cell migration and adhesion.

Results

We determined the influence of sodium concentration, pH, metabolic inhibitors, as well as the affinity f

SUBMITTER: Russo-Abrahao T 

PROVIDER: S-EPMC5802448 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature