Polymorphism in Tmem132d regulates expression and anxiety-related behavior through binding of RNA polymerase II complex.

ABSTRACT: TMEM132D is a candidate gene, where risk genotypes have been associated with anxiety severity along with higher mRNA expression in the frontal cortex of panic disorder patients. Concurrently, in a high (HAB) and low (LAB) trait anxiety mouse model, Tmem132d was found to show increased expression in the anterior cingulate cortex (aCC) of HAB as compared to LAB mice. To understand the molecular underpinnings underlying the differential expression, we sequenced the gene and found two single-nucleotide polymorphisms (SNPs) in the promoter differing between both lines which could explain the observed mRNA expression profiles using gene reporter assays. In addition, there was no difference in basal DNA methylation in the CpG Island that encompasses the HAB vs. LAB Tmem132d promoter region. Furth

SUBMITTER: Naik RR 

PROVIDER: S-EPMC5802467 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature