Supersaturation-controlled microcrystallization and visualization analysis for serial femtosecond crystallography.

ABSTRACT: Time-resolved serial femtosecond crystallography with X-ray free electron laser (XFEL) holds the potential to view fast reactions occurring at near-physiological temperature. However, production and characterization of homogeneous micron-sized protein crystals at high density remain a bottleneck, due to the lack of the necessary equipments in ordinary laboratories. We describe here supersaturation-controlled microcrystallization and visualization and analysis tools that can be easily used in any laboratory. The microcrystallization conditions of the influenza virus hemagglutinin were initially obtained with low reproducibility, which was improved by employing a rapid evaporation of hanging drops. Supersaturation-controlled microcrystallization was then developed in a vapor diffusion mode,

SUBMITTER: Lee DB 

PROVIDER: S-EPMC5803221 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature