SJP-L-5 inhibits HIV-1 polypurine tract primed plus-strand DNA elongation, indicating viral DNA synthesis initiation at multiple sites under drug pressure.

ABSTRACT: In a previous study the small molecule SJP-L-5 that inhibits HIV replication, has been shown to block uncoating of the viral capsid. Continued study showed that SJP-L-5 might hinder HIV capsid uncoating by blocking the completion of reverse transcription. However, to date, the mechanism has not been fully elucidated. Here, the effects of SJP-L-5 for reverse transcription were explored via quantitative PCR, DIG-labelled ELISA, fluorescent resonance energy transfer, and Southern blot assays. We also analyzed the resistance profile of this compound against reverse transcriptase. Our results show that SJP-L-5 preferentially inhibits PPT primed plus-strand DNA synthesis (EC₅₀ = 13.4 ± 3.0 μM) over RNA primed minus-strand DNA synthesis (EC₅₀ > 3,646 μM), resulting in format

SUBMITTER: Zhang XJ 

PROVIDER: S-EPMC5803243 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature