Transfusion strategy for weak D Type 4.0 based on RHD alleles and RH haplotypes in Tunisia.

ABSTRACT:

Background

With more than 460 RHD alleles, this gene is the most complex and polymorphic among all blood group systems. The Tunisian population has the largest known prevalence of weak D Type 4.0 alleles, occurring in one of 105 RH haplotypes. We aimed to establish a rationale for the transfusion strategy of weak D Type 4.0 in Tunisia.

Study design and methods

Donors were randomly screened for the serologic weak D phenotype. The RHD coding sequence and parts of the introns were sequenced. To establish the RH haplotype, the RHCE gene was tested for characteristic single-nucleotide positions.

Results

We determined all RHD alleles and the RH haplotypes coding for the serologic weak D phenotype among 13,431 Tunisian donations. A serologic weak D phenotype was found in 67

SUBMITTER: Ouchari M 

PROVIDER: S-EPMC5803429 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature