Parkin clearance of dysfunctional mitochondria regulates ROS levels and increases survival of human chondrocytes.

ABSTRACT:

Objective

Mitochondrial dysfunction, oxidative stress and chondrocyte death are important contributors to the development and pathogenesis of osteoarthritis (OA). In this study, we determined the expression and role of Parkin in the clearance of damaged/dysfunctional mitochondria, regulation of reactive oxygen species (ROS) levels and chondrocyte survival under pathological conditions.

Methods

Human chondrocytes were from the unaffected area of knee OA cartilage (n = 12) and were stimulated with IL-1β to mimic pathological conditions. Mitochondrial membrane depolarization and ROS levels were determined using specific dyes and flow cytometry. Autophagy was determined by Western blotting for ATG5, Beclin1, immunofluorescence staining and confocal microscopy. Gene expression wa

SUBMITTER: Ansari MY 

PROVIDER: S-EPMC5803469 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature