Comparative analytical utility of DNA derived from alternative human specimens for molecular autopsy and diagnostics.

ABSTRACT: Genetic testing and research have increased the demand for high-quality DNA that has traditionally been obtained by venipuncture. However, venous blood collection may prove difficult in special populations and when large-scale specimen collection or exchange is prerequisite for international collaborative investigations. Guthrie/FTA card-based blood spots, buccal scrapes, and finger nail clippings are DNA-containing specimens that are uniquely accessible and thus attractive as alternative tissue sources (ATS). The literature details a variety of protocols for extraction of nucleic acids from a singular ATS type, but their utility has not been systematically analyzed in comparison with conventional sources such as venous blood. Additionally, the efficacy of each protocol is often equated wi

SUBMITTER: Klassen TL 

PROVIDER: S-EPMC5803546 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature