Data Independent Analysis of IgG Glycoforms in Samples of Unfractionated Human Plasma.

ABSTRACT: Glycosylation regulates functional responses mediated by the interaction of IgG with their receptors. Multiple analytical methods have been designed for the determination of the IgG N-glycan microheterogeneity, including MS methods for the analysis of site specific glycoforms of IgG. However, measurement of low abundant glycoforms remains challenging in complex samples like serum without enrichment of the IgG. We present a workflow for quantitative analysis of site specific glycoforms of IgG based on data independent acquisition (DIA) of Y-ions generated under "minimal" fragmentation conditions. The adjusted collision induced dissociation (CID) conditions generate specific Y-ions in the yield of up to 60% precursor ion intensity. These selective fragments, measured in high resolution, impr

SUBMITTER: Sanda M 

PROVIDER: S-EPMC5803558 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature