MiR-422a suppresses SMAD4 protein expression and promotes resistance to muscle loss.

ABSTRACT:

Background

Loss of muscle mass and strength are important sequelae of chronic disease, but the response of individuals is remarkably variable, suggesting important genetic and epigenetic modulators of muscle homeostasis. Such factors are likely to modify the activity of pathways that regulate wasting, but to date, few such factors have been identified.

Methods

The effect of miR-422a on SMAD4 expression and transforming growth factor (TGF)-β signalling were determined by western blotting and luciferase assay. miRNA expression was determined by qPCR in plasma and muscle biopsy samples from a cross-sectional study of patients with chronic obstructive pulmonary disease (COPD) and a longitudinal study of patients undergoing aortic surgery, who were subsequently admitted to the in

SUBMITTER: Paul R 

PROVIDER: S-EPMC5803610 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature