Cathepsin K activity controls cardiotoxin-induced skeletal muscle repair in mice.

ABSTRACT:

Background

Cathepsin K (CatK) is a widely expressed cysteine protease that has gained attention because of its enzymatic and non-enzymatic functions in signalling. Here, we examined whether CatK-deficiency (CatK^-/- ) would mitigate injury-related skeletal muscle remodelling and fibrosis in mice, with a special focus on inflammation and muscle cell apoptosis.

Methods

Cardiotoxin (CTX, 20 μM/200 μL) was injected into the left gastrocnemius muscle of male wild-type (CatK^+/+ ) and CatK^-/- mice, and the mice were processed for morphological and biochemical studies.

Results

On post-injection Day 14, CatK deletion ameliorated muscle interstitial fibrosis and remodelling and performance. At an early time point (Day 3), CatK^-/- reduc

SUBMITTER: Ogasawara S 

PROVIDER: S-EPMC5803616 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature