Improvement of malaria diagnostic system based on acridine orange staining.

ABSTRACT: BACKGROUND:Rapid diagnosis of malaria using acridine orange (AO) staining and a light microscope with a halogen lamp and interference filter was deployed in some malaria-endemic countries. However, it has not been widely adopted because: (1) the lamp was weak as an excitation light and the set-up did not work well under unstable power supply; and, (2) the staining of samples was frequently inconsistent. METHODS:The halogen lamp was replaced by a low-cost, blue light-emitting diode (LED) lamp. Using a reformulated AO solution, the staining protocol was revised to make use of a concentration gradient instead of uniform staining. To evaluate this new AO diagnostic system, a pilot field study was conducted in the Lake Victoria basin in Kenya. RESULTS:Without staining failure, malaria infection

SUBMITTER: Kimura M 

PROVIDER: S-EPMC5804042 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature