Conventional and accelerated-solvent extractions of green tea (camellia sinensis) for metabolomics-based chemometrics.

ABSTRACT: Metabolomics has emerged as an important analytical technique for multiple applications. The value of information obtained from metabolomics analysis depends on the degree to which the entire metabolome is present and the reliability of sample treatment to ensure reproducibility across the study. The purpose of this study was to compare methods of preparing complex botanical extract samples prior to metabolomics profiling. Two extraction methodologies, accelerated solvent extraction and a conventional solvent maceration, were compared using commercial green tea [Camellia sinensis (L.) Kuntze (Theaceae)] products as a test case. The accelerated solvent protocol was first evaluated to ascertain critical factors influencing extraction using a D-optimal experimental design study. The accelerat

SUBMITTER: Kellogg JJ 

PROVIDER: S-EPMC5804813 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature