Dried Blood Spots for Measuring Vibrio cholerae-specific Immune Responses.

ABSTRACT:

Background

Vibrio cholerae causes over 2 million cases of cholera and 90,000 deaths each year. Serosurveillance can be a useful tool for estimating the intensity of cholera transmission and prioritizing populations for cholera control interventions. Current methods involving venous blood draws and downstream specimen storage and transport methods pose logistical challenges in most settings where cholera strikes. To overcome these challenges, we developed methods for determining cholera-specific immune responses from dried blood spots (DBS).

Methodology/principal findings

As conventional vibriocidal assay methods were unsuitable for DBS eluates from filter paper, we adopted a drop-plate culture method. We show that DBS collected from volunteers in South Sudan, and stored for

SUBMITTER: Iyer AS 

PROVIDER: S-EPMC5805362 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature