Analysis of airborne microbial communities using 16S ribosomal RNA: Potential bias due to air sampling stress.

ABSTRACT: A limited number of studies have been conducted to analyze ribosomal RNA (rRNA, present in the ribosome) in bioaerosol samples to identify currently or potentially active airborne microbes, although its genomic counterpart, the rRNA gene (on the chromosome) has been frequently targeted for airborne microbial community analysis. A knowledge gap still exists regarding whether the bioaerosol rRNA abundances are affected by the bioaerosol collection process. We investigated the effect of air sampling stress on the measurement and characterization of 16S rRNA for bioaerosols in the laboratory and field experiments using quantitative polymerase chain reaction (qPCR) and high-throughput sequencing techniques. In a laboratory study, known quantities of freshly grown Escherichia coli cells were spi

SUBMITTER: Zhen H 

PROVIDER: S-EPMC5805565 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature