Personalized Proteomics in Proliferative Vitreoretinopathy Implicate Hematopoietic Cell Recruitment and mTOR as a Therapeutic Target.

ABSTRACT:

Purpose

To profile vitreous cytokine expression of proliferative vitreoretinopathy (PVR) patients.

Design

Case-control study.

Methods

Liquid biopsies were collected from 2 groups: control subjects (n = 3) undergoing pars plana vitrectomy to remove an epiretinal membrane (ERM), and test subjects (n = 7) with varying degrees of PVR. A high-throughput cytokine screen measured expression of 200 cytokines. Cytokine expression patterns were prospectively validated in separate cohorts of control patients and those with PVR-A, PVR-B, and PVR-C (n = 10 for each group). Expression changes were evaluated by analysis of variance (significant P value < .05), hierarchical cluster algorithm, and pathway analysis, to identify candidate pathways for prospective studies.

Results

SUBMITTER: Roybal CN 

PROVIDER: S-EPMC5805631 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature