Probing the local lipid environment of the Rhodobacter sphaeroides cytochrome bc₁ and Synechocystis sp. PCC 6803 cytochrome b₆f complexes with styrene maleic acid.

ABSTRACT: Intracytoplasmic vesicles (chromatophores) in the photosynthetic bacterium Rhodobacter sphaeroides represent a minimal structural and functional unit for absorbing photons and utilising their energy for the generation of ATP. The cytochrome bc₁ complex (cytbc₁) is one of the four major components of the chromatophore alongside the reaction centre-light harvesting 1-PufX core complex (RC-LH1-PufX), the light-harvesting 2 complex (LH2), and ATP synthase. Although the membrane organisation of these complexes is known, their local lipid environments have not been investigated. Here we utilise poly(styrene-alt-maleic acid) (SMA) co-polymers as a tool to simultaneously determine the local lipid environments of the RC-LH1-PufX, LH2 and cytbc₁ complexes. SMA has pr

SUBMITTER: Swainsbury DJK 

PROVIDER: S-EPMC5805856 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature