Contribution of low-temperature single-molecule techniques to structural issues of pigment-protein complexes from photosynthetic purple bacteria.

ABSTRACT: As the electronic energies of the chromophores in a pigment-protein complex are imposed by the geometrical structure of the protein, this allows the spectral information obtained to be compared with predictions derived from structural models. Thereby, the single-molecule approach is particularly suited for the elucidation of specific, distinctive spectral features that are key for a particular model structure, and that would not be observable in ensemble-averaged spectra due to the heterogeneity of the biological objects. In this concise review, we illustrate with the example of the light-harvesting complexes from photosynthetic purple bacteria how results from low-temperature single-molecule spectroscopy can be used to discriminate between different structural models. Thereby the low-temp

SUBMITTER: Lohner A 

PROVIDER: S-EPMC5805968 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature