Selective Radical Trifluoromethylation of Native Residues in Proteins.

ABSTRACT: The incorporation of fluorine can not only significantly facilitate the study of proteins but also potentially modulate their function. Though some biosynthetic methods allow global residue-replacement, post-translational fluorine incorporation would constitute a fast and efficient alternative. Here, we reveal a mild method for direct protein radical trifluoromethylation at native residues as a strategy for symmetric-multifluorine incorporation on mg scales with high recoveries. High selectivity toward tryptophan residues enhanced the utility of this direct trifluoromethylation technique allowing ready study of fluorinated protein constructs using ¹⁹F-NMR.

SUBMITTER: Imiolek M 

PROVIDER: S-EPMC5806083 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature