Generation of Immunity against Pathogens via Single-Domain Antibody-Antigen Constructs.

ABSTRACT: mAbs specific for surface proteins on APCs can serve as Ag-delivery vehicles that enhance immunogenicity. The practical use of such constructs is limited by the challenge of expressing and modifying full-sized mAbs. We generated single-domain Ab fragments (VHHs) specific for class II MHC (MHCII), CD11b, and CD36. VHH sequences were modified by inclusion of a C-terminal sortase motif to allow site-specific conjugation with various Ag payloads. We tested T cell activation using VHHs that target distinct APC populations; anti-MHCII adducts elicited strong activation of CD4⁺ T cells, whereas anti-CD11b showed CD8⁺ T cell activation superior to targeting via MHCII and CD36. Differences in Ag presentation among constructs were unrelated to dendritic cell subtype or routing

SUBMITTER: Duarte JN 

PROVIDER: S-EPMC5806123 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature