Easy and fast method for expression and native extraction of Plasmodium vivax Duffy binding protein fragments.

ABSTRACT: BACKGROUND:The Plasmodium vivax Duffy binding protein (PvDBP) has been the most studied ligand binding human reticulocytes to date. This molecule has a cysteine-rich domain in region II (RII) which has been used as control for evaluating the target cell binding activity of several parasite molecules. However, obtaining rPvDBP-RII in a soluble form using the Escherichia coli expression system usually requires laborious and time-consuming steps for recovering the molecule's structure and function, considering it is extracted from inclusion bodies. The present study describes an easy and fast method for expressing and obtaining several PvDBP fragments which should prove ideal for use in protein-cell interaction assays. RESULTS:Two PvDBP encoding regions (rii and riii/v) were cloned in pEXP5-C

SUBMITTER: Moreno-Perez DA 

PROVIDER: S-EPMC5806264 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature