A copper-controlled RNA interference system for reversible silencing of target genes in Trichoderma reesei.

ABSTRACT:

Background

Trichoderma reesei is a primary lignocellulosic enzyme producer in industry. However, the mechanisms underlying cellulase synthesis as well as other physiological processes are insufficiently understood partly due to the sophisticated process for its genetic manipulation. Target gene knockdown by RNA interference (RNAi) is a powerful tool for genetic research and biotechnology in eukaryotes including filamentous fungi. Previously reported RNAi system in T. reesei was either uncontrollable or only applicable in certain nutrition state.

Results

In the present study, we incorporated the copper-responsive tcu1 promoter into an RNAi-mediated silencing system to develop a controllable RNAi-mediated silencing system in T. reesei. As the proof-

SUBMITTER: Wang L 

PROVIDER: S-EPMC5806297 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature