Rapid and ultra-sensitive quantitation of disease-associated α-synuclein seeds in brain and cerebrospinal fluid by αSyn RT-QuIC.

ABSTRACT: The diagnosis and treatment of synucleinopathies such as Parkinson disease and dementia with Lewy bodies would be aided by the availability of assays for the pathogenic disease-associated forms of α-synuclein (αSyn^D) that are sufficiently sensitive, specific, and practical for analysis of accessible diagnostic specimens. Two recent αSyn^D seed amplification tests have provided the first prototypes for ultrasensitive and specific detection of αSyn^D in patients' cerebrospinal fluid. These prototypic assays require 5-13 days to perform. Here, we describe an improved α-synuclein real time quaking-induced conversion (αSyn RT-QuIC) assay that has similar sensitivity and specificity to the prior assays, but can be performed in 1-2 days with quantitation. Blinded an

SUBMITTER: Groveman BR 

PROVIDER: S-EPMC5806364 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature