Identification and characterization of influenza A viruses in selected domestic animals in Kenya, 2010-2012.

ABSTRACT:

Background

Influenza A virus subtypes in non-human hosts have not been characterized in Kenya. We carried out influenza surveillance in selected domestic animals and compared the virus isolates with isolates obtained in humans during the same period.

Methods

We collected nasal swabs from pigs, dogs and cats; oropharyngeal and cloacal swabs from poultry; and blood samples from all animals between 2010 and 2012. A standardized questionnaire was administered to farmers and traders. Swabs were tested for influenza A by rtRT-PCR, virus isolation and subtyping was done on all positive swabs. All sera were screened for influenza A antibodies by ELISA, and positives were evaluated by hemagglutination inhibition (HI). Full genome sequencing was done on four selected pig virus isolate

SUBMITTER: Munyua P 

PROVIDER: S-EPMC5806879 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature