Unique molecular events during reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs) at naive state.

ABSTRACT: Derivation of human naïve cells in the ground state of pluripotency provides promising avenues for developmental biology studies and therapeutic manipulations. However, the molecular mechanisms involved in the establishment and maintenance of human naïve pluripotency remain poorly understood. Using the human inducible reprogramming system together with the 5iLAF naïve induction strategy, integrative analysis of transcriptional and epigenetic dynamics across the transition from human fibroblasts to naïve iPSCs revealed ordered waves of gene network activation sharing signatures with those found during embryonic development from late embryogenesis to pre-implantation stages. More importantly, Transcriptional analysis showed a significant transient reactivation of transcripts with 8-cell-stag

SUBMITTER: Wang Y 

PROVIDER: S-EPMC5807049 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature