Performance of the BioPlex 2200 HIV Ag-Ab assay for identifying acute HIV infection.

ABSTRACT:

Background

Assays that detect HIV antigen (Ag) and antibody (Ab) can be used to screen for HIV infection.

Objectives

To compare the performance of the BioPlex 2200 HIV Ag-Ab assay and two other Ag/Ab combination assays for detection of acute HIV infection.

Study design

Samples were obtained from 24 individuals (18 from the US, 6 from South Africa); these individuals were classified as having acute infection based on the following criteria: positive qualitative RNA assay; two negative rapid tests; negative discriminatory test. The samples were tested with the BioPlex assay, the ARCHITECT HIV Ag/Ab Combo test, the Bio-Rad GS HIV Combo Ag-Ab EIA test, and a viral load assay.

Results

Twelve (50.0%) of 24 samples had RNA detected only ( > 40 to 13,476 copies/mL). Te

SUBMITTER: Eshleman SH 

PROVIDER: S-EPMC5807223 | biostudies-literature | 2018 Feb - Mar

REPOSITORIES: biostudies-literature