A Mathematical Model for Enzyme Clustering in Glucose Metabolism.

ABSTRACT: We have recently demonstrated that the rate-limiting enzymes in human glucose metabolism organize into cytoplasmic clusters to form a multienzyme complex, the glucosome, in at least three different sizes. Quantitative high-content imaging data support a hypothesis that the glucosome clusters regulate the direction of glucose flux between energy metabolism and building block biosynthesis in a cluster size-dependent manner. However, direct measurement of their functional contributions to cellular metabolism at subcellular levels has remained challenging. In this work, we develop a mathematical model using a system of ordinary differential equations, in which the association of the rate-limiting enzymes into multienzyme complexes is included as an essential element. We then demonstrate that o

SUBMITTER: Jeon M 

PROVIDER: S-EPMC5807315 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature