Microfluidic channel optimization to improve hydrodynamic dissociation of cell aggregates and tissue.

ABSTRACT: Maximizing the speed and efficiency at which single cells can be liberated from tissues would dramatically advance cell-based diagnostics and therapies. Conventional methods involve numerous manual processing steps and long enzymatic digestion times, yet are still inefficient. In previous work, we developed a microfluidic device with a network of branching channels to improve the dissociation of cell aggregates into single cells. However, this device was not tested on tissue specimens, and further development was limited by high cost and low feature resolution. In this work, we utilized a single layer, laser micro-machined polyimide film as a rapid prototyping tool to optimize the design of our microfluidic channels to maximize dissociation efficiency. This resulted in a new design with sm

SUBMITTER: Qiu X 

PROVIDER: S-EPMC5807353 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature