Raman spectroscopic imaging for quantification of depth-dependent and local heterogeneities in native and engineered cartilage.

ABSTRACT: Articular cartilage possesses a remarkable, mechanically-robust extracellular matrix (ECM) that is organized and distributed throughout the tissue to resist physiologic strains and provide low friction during articulation. The ability to characterize the make-up and distribution of the cartilage ECM is critical to both understand the process by which articular cartilage undergoes disease-related degeneration and to develop novel tissue repair strategies to restore tissue functionality. However, the ability to quantitatively measure the spatial distribution of cartilage ECM constituents throughout the tissue has remained a major challenge. In this experimental investigation, we assessed the analytical ability of Raman micro-spectroscopic imaging to semi-quantitatively measure the distributi

SUBMITTER: Albro MB 

PROVIDER: S-EPMC5807411 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature