Genome-Wide Bimolecular Fluorescence Complementation-Based Proteomic Analysis of Toxoplasma gondii ROP18's Human Interactome Shows Its Key Role in Regulation of Cell Immunity and Apoptosis.

ABSTRACT: Toxoplasma gondii rhoptry protein ROP18 (TgROP18) is a key virulence factor secreted into the host cell during invasion, where it modulates the host cell response by interacting with its host targets. However, only a few TgROP18 targets have been identified. In this study, we applied a high-throughput protein-protein interaction (PPI) screening in human cells using bimolecular fluorescence complementation (BiFC) to identify the targets of Type I strain ROP18 (ROP18_I) and Type II strain ROP18 (ROP18_II). From a pool of more than 18,000 human proteins, 492 and 141 proteins were identified as the targets of ROP18_I and ROP18_II, respectively. Gene ontology, search tool for the retrieval of interacting genes/proteins PPI network, an

SUBMITTER: Xia J 

PROVIDER: S-EPMC5807661 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature