Effect of heat shock on hot water plumbing microbiota and Legionella pneumophila control.

ABSTRACT:

Background

Heat shock is a potential control strategy for Legionella pneumophila in hot water plumbing systems. However, it is not consistently effective, with little understanding of its influence on the broader plumbing microbiome. Here, we employed a lab-scale recirculating hot water plumbing rig to compare the pre- and post-"heat shock" (i.e., 40 → 60 → 40 °C) microbiota at distal taps. In addition, we used a second plumbing rig to represent a well-managed system at 60 °C and conducted a "control" sampling at 60 °C, subsequently reducing the temperature to 40 °C to observe the effects on Legionella and the microbiota under a simulated "thermal disruption" scenario.

Results

According to 16S rRNA gene amplicon sequencing, in the heat shock scenario, there was no significan

SUBMITTER: Ji P 

PROVIDER: S-EPMC5807837 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature