Coupling the recombineering to Cre-lox system enables simplified large-scale genome deletion in Lactobacillus casei.

ABSTRACT:

Background

Lactobacillus casei is widely used in the dairy and pharmaceutical industries and a promising candidate for use as cell factories. Recently, genome sequencing and functional genomics provide the possibility for reducing L. casei genome. However, it was still limited by the inefficient and laborious genome deletion methods.

Results

Here, we proposed a genome minimization strategy based on LCABL_13040-50-60 recombineering and Cre-lox site-specific recombination system in L. casei. The LCABL_13040-50-60 recombineering system was used to introduce two lox sites (lox66 and lox71) into 5' and 3' ends of the targeted region. Subsequently, the targeted region was excised by Cre recombinase. The robustness of the strategy was demonstrated by single-deletion of a nonessenti

SUBMITTER: Xin Y 

PROVIDER: S-EPMC5808424 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature