SMURF1 facilitates estrogen receptor ɑ signaling in breast cancer cells.

ABSTRACT:

Background

Estrogen receptor alpha (ER alpha) is expressed in the majority of breast cancers and promotes estrogen-dependent cancer progression. ER alpha positive breast cancer can be well controlled by ER alpha modulators, such as tamoxifen. However, tamoxifen resistance is commonly observed by altered ER alpha signaling. Thus, further understanding of the molecular mechanisms, which regulates ER alpha signaling, is important to improve breast cancer therapy.

Methods

SMURF1 and ER alpha protein expression levels were measured by western blot, while the ER alpha target genes were measured by real-time PCR. WST-1 assay was used to measure cell viability; the xeno-graft tumor model were used for in vivo study. RNA sequencing was analyzed by Ingenuity Pathway Analysis. Identifi

SUBMITTER: Yang H 

PROVIDER: S-EPMC5808446 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature