FLEXITau: Quantifying Post-translational Modifications of Tau Protein in Vitro and in Human Disease.

ABSTRACT: Tauopathies, including Alzheimer's disease (AD), are associated with the aggregation of modified microtubule associated protein tau. This pathological state of tau is often referred to as "hyperphosphorylated". Due to limitations in technology, an accurate quantitative description of this state is lacking. Here, a mass spectrometry-based assay, FLEXITau, is presented to measure phosphorylation stoichiometry and provide an unbiased quantitative view of the tau post-translational modification (PTM) landscape. The power of this assay is demonstrated by measuring the state of hyperphosphorylation from tau in a cellular model for AD pathology, mapping, and calculating site occupancies for over 20 phosphorylations. We further employ FLEXITau to define the tau PTM landscape present in AD post-mor

SUBMITTER: Mair W 

PROVIDER: S-EPMC5808556 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature