Dynamics and number of trans-SNARE complexes determine nascent fusion pore properties.

ABSTRACT: The fusion pore is the first crucial intermediate formed during exocytosis, yet little is known about the mechanisms that determine the size and kinetic properties of these transient structures. Here, we reduced the number of available SNAREs (proteins that mediate vesicle fusion) in neurons and observed changes in transmitter release that are suggestive of alterations in fusion pores. To investigate these changes, we employed reconstituted fusion assays using nanodiscs to trap pores in their initial open state. Optical measurements revealed that increasing the number of SNARE complexes enhanced the rate of release from single pores and enabled the escape of larger cargoes. To determine whether this effect was due to changes in nascent pore size or to changes in stability, we developed an

SUBMITTER: Bao H 

PROVIDER: S-EPMC5808578 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature