Positive charge in the n-region of the signal peptide contributes to efficient post-translational translocation of small secretory preproteins.

ABSTRACT: Increasing evidence indicates that many small secretory preproteins can undergo post-translational translocation across the membrane of the endoplasmic reticulum. Although the cellular machinery involved in post-translational translocation of small secretory preproteins has begun to be elucidated, the intrinsic signals contained within these small secretory preproteins that contribute to their efficient post-translational translocation remain unknown. Here, we analyzed the eukaryotic secretory proteome and discovered the small secretory preproteins tend to have a higher probability to harbor the positive charge in the n-region of the signal peptide (SP). Eliminating the positive charge of the n-region blocked post-translational translocation of newly synthesized preproteins and selectively

SUBMITTER: Guo H 

PROVIDER: S-EPMC5808753 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature